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Publikation Nr. 736 - Details

New HIV-protease assays applying self-quenching peptide substrates in combination with time-resolved fluorescence single-molecule spectroscopy

Staudt, T., Kräusslich, H. G., Knemeyer, J. P. & Marmé, N. (2007). New HIV-protease assays applying self-quenching peptide substrates in combination with time-resolved fluorescence single-molecule spectroscopy, International Journal of Environmental Analytical Chemistry , 2007(87), 731-743.

Artikel

URL: https://www.researchgate.net/publication/233359623_New_HIV-protease_assays_applying_self-quenching_peptide_substrates_in_combination_with_time-resolved_fluorescence_single-molecule_spectroscopy

Abstract
This work describes the optimization and adoption of an assay system for the Human Immunodeficiency Virus (HIV)-protease, whose inhibition plays a central role in HIV therapy. The HIV-protease, which is an essential enzyme during viral maturation, has a specific cleavage site of eight amino acid residues (SQNY*PIV). Adding two amino acid residues at the N-terminus and enclosing the resulting sequence by a dye-labelled lysine residue and a tryptophan residue leads to the substrate (K(dye)CGSQNY*P

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Sprache:
Art der Begutachtung:	Peer Review(Double-blind peer review)
Print:	Ja
Online:	Ja, ohne Open Access
Datenmedium:	Keine Angabe