Knemeyer, J. P., Marmé, N. & Sauer, M. (2000). Probes for detection of specific DNA Sequences at the single-molecule level, Analytical chemistry, 2000(72), 3717-3724.
Abstract A method has been developed for highly sensitive detection of specific DNA sequences in a homogeneous assay using labeled oligonucleotide molecules in combination with single-molecule photon burst counting and identification. The fluorescently labeled oligonucleotides are called smart probes because they report the presence of complementary target sequences by a strong increase in fluorescence intensity. The smart probes consist of a fluorescent dye attached at the terminus of a hairpin oligonuc