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Publikation Nr. 718 - Details

Marmé, N., Knemeyer, J. P., Sauer, M. & Wolfrum, J. (2003). Inter-and intramolecular fluorescence quenching of organic dyes by tryptophan, Bioconjugate chemistry, 2003(14), 1133-1139.


Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655, ATTO 680, and the oxazine derivative MR 121 are strongly quenched almost exclusively by tryptophan due to the formation of weak or nonfluorescent ground-state complexes

Art der Begutachtung: Peer Review(Double-blind peer review)
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Online: Ja, ohne Open Access
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