Park, C. W., Knemeyer, J. P., Marmé, N., Möller, M., Spatz, J., Wolfrum, J. & Sauer, M. (2004). Accurate delivery of single biomolecules by polyethylene glycol coated submicrometer pipettes, Chemical Physics, 2004(1), 105-110.
Abstract There is a great interest to quantitatively manipulate, separate, and deliver low concentrations or even single biomolecules in submicrometer-sized channels. We report the use of cross-linked four-star polyethylene glycol (PEG) as a new coating technique for microchannels. PEG-coating efficiently reduces the electroosmotic flow (EOF) and non-specific adsorption to the glass walls. Our results demonstrate that individual fluorescently labeled hydrophobic cell adhesion proteins (fibronectin molecu