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Publikation Nr. 720 - Details Marmé, N., Knemeyer, J. P., Sauer, M. & Wolfrum, J. (2002). Specific detection of proteolytic enzymes, Patent: US 7329505 B2. URL: https://www.researchgate.net/publication/316241223_Specific_detection_of_proteolytic_enzymes Abstract Specific detection of proteolytic enzymes is achieved by extinguishing dye fluorescence by the amino acid tryptophan. Ttyptophan is disposed on one side of the cutting site of a proteolytic enzyme while an amino acid marked with a dye is arranged on the other side. Extinction of fluorescence occurs prior to enzyme cutting. Spatial separation of the tryptophan and the dye takes place after cutting, whereby fluorescence extinction does not occur. The dye can then fluoresce and a signal increase oc Attribute:
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