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Publikation Nr. 721 - Details

Neuweiler, H., Herten, D. P., Marmé, N., Knemeyer, J. P., Piestert, O., Tinnefeld, P. & Sauer, M. (2004). Probing conformational dynamics by photoinduced electron transfer, Proceedings of SPIE, 2004(5322), 8-19.

DOI: 10.1117/12.531167

We demonstrate how photoinduced electron transfer (PET) reactions can be successfully applied to monitor conformational dynamics in individual biopolymers. Single-pair fluorescence resonance energy transfer (FRET) experiments are ideally suited to study conformational dynamics occurring on the nanometer scale, e.g. during protein folding or unfolding. In contrast, conformational dynamics with functional significance, for example occurring in enzymes at work, often appear on much smaller spatial

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