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Publikation Nr. 723 - Details

Marmé, N., Knemeyer, J. P., Wolfrum, J. & Sauer, M. (2004). Highly Sensitive Protease Assay Using Fluorescence Quenching of Peptide Probes Based on Photoinduced Electron Transfer, Angewandte Chemie International Edition, 2004(43), 798-801.


Making the cut and then detecting it: Short fluorophore-labeled peptide substrates (see scheme) show strong fluorescence quenching due to electron transfer from a tryptophan residue (blue) to the dye (gray, red). Upon specific cleavage of the peptide by proteolytic enzymes, quenching is prevented and the fluorescence intensity increases. The method can be used to assay proteolytic enzymes with detection limits in the picomolar range.

Art der Begutachtung: Peer Review(Double-blind peer review)
Print: Ja
Online: Ja, ohne Open Access
Datenmedium: Nein