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Publikation Nr. 724 - Details

Schäfer, G., Müller, M., Häfner, B., Habl, G., Nolte, O., Marmé, N. & Knemeyer, J. P. (2005). Self-quenching DNA probes based on aggregation of fluorescent dyes, Proceedings of SPIE - The International Society for Optical Engineering, 2005(5704), 30-39.


Here we present a novel class of self-quenching, double-labeled DNA probes based on the formation of non fluorescent H-type dye dimers. We therefore investigated the aggregation behavior of the red-absorbing oxazine derivative MR121 and found a dimerization constant of about 3000 M-1. This dye was successfully used to develop hairpin-structured as well as linear self-quenching DNA probes that report the presence of the target DNA by an increase of the fluorescence intensity by a factor of 3 to 1

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