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Publikation Nr. 725 - Details

Staudt, T., Knemeyer, L., Kräusslich, H. G., Knemeyer, J. P. & Marmé, N. (2005). Novel fluorescently labeled enzyme substrates for the sensitive detection of HIV-protease, Proceedings of SPIE - The International Society for Optical Engineering, 2005(5704), 112-119.


In this paper we applied the efficient fluorescence quenching of the red-absorbing oxazine derivative MR121 by the amino acid tryptophan to develop a new fluorescence based enzyme assay that can be used for detection of exopeptidases and endopeptidases. Therefore, we developed peptide substrates labeled with only one chromophore, which is quenched by a neighbored tryptophan residue via photoinduced electron transfer. The specific cleavage site for the target enzyme is located between the chromop

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