Marmé, N., Friedrich, A., Müller, M., Nolte, O., Wolfrum, J., Hoheisel, J. D., Sauer, M. & Knemeyer, J. P. (2006). Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy, Nucleic Acids Research, 2006(34), 90-100.
Abstract We demonstrate the specific identification of single nucleotide polymorphism (SNP) responsible for rifampicin resistance of Mycobacterium tuberculosis applying fluorescently labeled DNA-hairpin structures (smart probes) in combination with single-molecule fluorescence spectroscopy. Smart probes are singly labeled hairpin-shaped oligonucleotides bearing a fluorescent dye at the 5′ end that is quenched by guanosine residues in the complementary stem. Upon hybridization to target sequences, a