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Publikation Nr. 733 - Details Knemeyer, J. P., Marmé, N. & Hoheisel, J. D. (2007). Spectrally resolved fluorescence lifetime imaging microscopy (SFLIM) - An appropriate method for imaging single molecules in living cells, Analytical and Bioanalytical Chemistry, 2007(387), 37-40. URL: https://www.researchgate.net/publication/6620590_Spectrally_resolved_fluorescence_lifetime_imaging_microscopy_SFLIM_-_An_appropriate_method_for_imaging_single_molecules_in_living_cells Abstract Spectrally Resolved Fluorescence Lifetime Imaging Microscopy (SFLIM), that uses a standard confocal microscope for imaging single molecules in living cells was presented. The fluorescence lifetime images were generated by recording the signals from two avalanche photodiodes by time-correlated single-photon counting interface card. The image signal data to determine monoexponential fluorescence lifetimes from a low photon statistics, was analyzed by applying a maximum likelihood estimator (MLE) a Attribute:
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