Friedrich, A., Habl, G., Sauer, M., Wolfrum, J., Hoheisel, J. D., Marmé, N. & Knemeyer, J. P. (2007). New hairpin-structured DNA probes: Alternatives to classical molecular beacons, Proceedings of SPIE - The International Society for Optical Engineering, 2007(6444), 111-117.
Abstract In this article we report on two different classes of self-quenching hairpin-structured DNA probes that can be used as alternatives to Molecular Beacons. Compared to other hairpin-structured DNA probes, the so-called smart probes are labeled with only one extrinsic dye. The fluorescence of this dye is efficiently quenched by intrinsic guanine bases via a photo-induced electron transfer reaction in the closed hairpin. After hybridization to a target DNA, the distance between dye and the guanines