Friedrich, A., Hoheisel, J. D., Marmé, N. & Knemeyer, J. P. (2007). DNA-probes for the highly sensitive identification of single nucleotide polymorphism using single-molecule spectroscopy, FEBS Letters, 2007(581), 1644-1648.
Abstract This article presents a new, highly sensitive method for the identification of single nucleotide polymorphisms (SNPs) in homogeneous solutions using fluorescently labeled hairpin-structured oligonucleotides (smart probes) and fluorescence single-molecule spectroscopy. While the hairpin probe is closed, fluorescence intensity is quenched due to close contact between the chromophore and several guanosine residues. Upon hybridization to the respective target SNP sequence, contact is lost and the fl