Friedrich, A., Müller, M., Nolte, O., Wolfrum, J., Sauer, M., Hoheisel, J. D., Knemeyer, J. P. & Marmé, N. (2008). Highly specific identification of single nucleic polymorphism in M. tuberculosis using smart probes and single-molecule fluorescence spectroscopy in combination with blocking oligonucleotides, Proceedings of SPIE - The International Society for Optical Engineering, 2008(6867), 15-22.
Abstract In this article we present a method for the highly specific identification of single nucleotide polymorphism (SNP) responsible for rifampicin resistance of Mycobacterium tuberculosis. This approach applies fluorescently labeled hairpin-structured oligonucleotides (smart probes) and confocal single-molecule fluorescence spectroscopy. Smart probes are fluorescently labeled at the 5'-end. The dye's fluorescence is quenched in the closed hairpin conformation due to close proximity of the g