Marmé, N., Lee, H., Friedrich, A., Park, C. W., Fiore, J., Nesbitt, D. & Knemeyer, J. P. (2008). Suppressing nonspecific adsorption of proteins on the single-molecular level, Proceedings of SPIE - The International Society for Optical Engineering, 2008(6862), 111-117.
Abstract Avoiding nonspecific surface adsorption is a crucial and often challenging issue in many single-molecule studies and analytical applications. In this work, we investigated glass surfaces coated with cross-linking star-shaped polyethylene glycol (4-arm PEG) and demonstrated that this coating can be used for effective suppression of nonspecific protein binding, such as streptavidin. Single-molecule fluorescence images show that only a few molecules remain nonspecifically bound to surfaces treated